Journal: Annals of Medicine and Surgery

Article Title: Da-yuan-yin decoction ameliorates inflammatory injury in severe pancreatitis by protecting intestinal mucosal barrier and immune function and preventing intestinal dysbiosis

doi: 10.1097/MS9.0000000000004873

Figure Lengend Snippet: Serum interleukin (IL)-1β and IL-10 expression levels (pg/ml) and cluster of differentiation (CD)3 + , CD4 + , and CD8 + cells from five ileal Peyer’s patches in the sham operation (SO), severe acute pancreatitis (SAP), and da-yuan-yin (DYY) + SAP groups, respectively. Serum IL-1β and IL-10 levels in the SAP and DYY + SAP groups were significantly higher than those in the SO group at 24, 48, and 72 h after surgery (all P < 0.05). Serum IL-1β and IL-10 levels in the DYY + SAP group were significantly lower than those in the SAP group 72 h postoperatively ( t = 4.37, P = 0.003; t = 2.41, P = 0.04, respectively). Levels of CD3 + , CD4 + , and CD8 + cells from ileal Peyer’s patches in the SAP and DYY + SAP groups decreased with time post-surgery. At 72 h postoperatively, the DYY + SAP group had significantly higher levels of CD3 + , CD4 + , and CD8 + cells than the SAP group ( t = 2.58, P = 0.036; t = 2.78, P = 0.027; t = 2.77, P = 0.028, respectively). There were no significant differences in early or late apoptosis in ileal Peyer’s patches 24, 48, and 72 h postoperatively in the SO, SAP, and DYY + SAP groups.

Article Snippet: Single-cell suspension is stained with the following antibodies: Live/Dead Fixable Violet Dead Cell (Cat# 2549272, Invitrogen), anti-Rat CD3 (Cat# G4.18, MultiSciences Biotech Co., Ltd), and anti-Rat CD4 (Cat# OX35, MultiSciences).

Techniques: Expressing

Journal: BMC Immunology

Article Title: CD30 knockout attenuates experimental colitis by reducing inflammatory cytokine production

doi: 10.1186/s12865-026-00816-w

Figure Lengend Snippet: CD30 Knockdown Suppresses Th1 Cell Differentiation In Vitro. A Representative flow cytometry plot showing purity of isolated naive CD4 + T cells (CD62L vs. CD44 gated on CD4 + cells). Average purity: 97.2 ± 1.3%. B CD30 mRNA knockdown efficiency by qRT-PCR ( n = 6 per group). C Western blot bands of protein expression in each group. D CD30 protein knockdown efficiency by Western blot with quantification ( n = 6 per group). E Representative flow cytometry plots of IFN-γ + cells among CD4 + T cells (gated on CD4 + population). F Quantification of IFN-γ + cell percentages among CD4 + T cells ( n = 6 per group). G Quantification of total CD4 + T cell percentages among all live cells ( n = 6 per group). Data are presented as mean ± SD. ** P < 0.01 vs. Control group; or ## P < 0.01 vs. si-NC group. All experiments were independently repeated three times with similar results

Article Snippet: Single-cell suspensions were prepared by mechanical dissociation through 70 μm cell strainers (BD Biosciences, San Jose, CA, USA), and naive CD4 + T cells (CD4 + CD25-CD44lowCD62Lhigh) were isolated using magnetic-activated cell sorting (MACS) with a Naive CD4 + T Cell Isolation Kit (Cat. No. 130-104-453; Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol.

Techniques: Knockdown, Cell Differentiation, In Vitro, Flow Cytometry, Isolation, Quantitative RT-PCR, Western Blot, Expressing, Control

Journal: BMC Immunology

Article Title: CD30 knockout attenuates experimental colitis by reducing inflammatory cytokine production

doi: 10.1186/s12865-026-00816-w

Figure Lengend Snippet: CD30 Promotes Th1 Differentiation Under Inflammatory Conditions. A Representative flow cytometry plots of IFN-γ + cells among CD4 + T cells (gated on CD4 + population) in PBS, LPS, si-NC + LPS, si-CD30 + PBS, and si-CD30 + LPS groups. B Quantification of IFN-γ + cell percentages among CD4 + T cells ( n = 6 per group). C mRNA expression of T-bet by qRT-PCR ( n = 6 per group). D Western blot bands of protein expression in each group. E mRNA expression of IFN-γ by qRT-PCR ( n = 6 per group). F-H Western blot analysis of T-bet ( F ), total STAT4 ( G ), and p-STAT4 ( H ) with quantification ( n = 6 per group). I-J Secreted concentrations of IFN-γ ( I ) and IL-2 ( J ) by ELISA (pg/ml) ( n = 6 per group). Data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 vs. PBS group; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. LPS group; & P < 0.05, && P < 0.01, &&& P < 0.001 vs. si-NC + LPS group. All experiments were independently repeated three times with similar results

Article Snippet: Single-cell suspensions were prepared by mechanical dissociation through 70 μm cell strainers (BD Biosciences, San Jose, CA, USA), and naive CD4 + T cells (CD4 + CD25-CD44lowCD62Lhigh) were isolated using magnetic-activated cell sorting (MACS) with a Naive CD4 + T Cell Isolation Kit (Cat. No. 130-104-453; Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol.

Techniques: Flow Cytometry, Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay